ASTM D4412-1984(2009) 水和水形成沉积物中硫酸盐还原菌的标准试验方法

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【英文标准名称】:StandardTestMethodsforSulfate-ReducingBacteriainWaterandWater-FormedDeposits
【原文标准名称】:水和水形成沉积物中硫酸盐还原菌的标准试验方法
【标准号】:ASTMD4412-1984(2009)
【标准状态】:现行
【国别】:美国
【发布日期】:1984
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D19.24
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:
【英文主题词】:Bacteria/bacterialcontrol--water;Most-probable-numbertechnique;Pathogens--water;Sulfatecontent--water;Thermophilicsulfate-reducingbacteria;Water-formeddeposits
【摘要】:Sulfate-reducingbacteriaarewidelydistributedinmarineandfreshwatermudswhich,inconsequence,frequentlyareladenwiththehydrogensulfideproducedbytheseorganismsduringdissimilatorysulfatereduction.IthasbeenreportedthatDesulfovibriocanformasmuchas10gofsulfideperlitreduringactivemultiplication.Sulfate-reducingbacteriacancausetheexternalorinternalcorrosionofwaterorwastewaterpipelinesandpipelinesforpetroleumandnaturalgas.Theformationofgalvaniccellsbymassivegrowthofsulfate-reducingbacteriaundersuitableconditionsmakesthecorrosionmuchworsethanjusttheeffectofthehydrogensulfideonthemetalorconcrete.1.1Thesetestmethodscovertheprocedureforthedetectionandenumerationbythemostprobablenumber(MPN)techniqueofsulfate-reducingbacteriainwaterorwater-formeddeposits.1.2Twomediapreparationsareprovided.MediumAwhichispreparedwithreagentgradewater,andMediumBwhichispreparedusingthewatertobesampledasthewatersource.MediumBisofferedforthosespecialconditionswheresulfate-reducingbacterialstrainshaveadaptedtoatypicalnon-freshwaterenvironment.1.3Fortheisolationandenumerationofthermophilicsulfate-reducingbacteriaencounteredinwatersassociatedwithoilandgasproduction,allbroths,dilutionblanks,andincubationsmustbemaintainedattemperaturesofatleast45x00B0;Candpreferablywithin5x00B0;Catthesampletemperature.1.4Thesensitivityofthesetestmethodscanbeincreasedbypurgingthedilutionblanksandtubesofmediawithnitrogenimmediatelypriortouse.1.5TheanalystshouldbeawarethatadequatecollaborativedataforprecisionandbiasstatementsasrequiredbyPracticeD2777arenotprovided.SeeSection11fordetails.1.6ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.7Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:Z16
【国际标准分类号】:07_100_20
【页数】:3P.;A4
【正文语种】:英语